• 85.5 gm sucrose (99.5% purity i.e. from SIGMA - S-9378);
• 11.76 gm C₆H₅O₇Na₃ - 2H₂O (dihydrate trisodium citrate; 99% pure i.e. from SIGMA - C-3434);
• dissolve in distilled water to 800 ml;
• add 50 ml DMSO (dimethyl sulfoxide; 99.9% pure i.e. from SIGMA D-8779);
• add distilled water to 1 litre;
• adjust to pH 7.6 using a 1.0 molar solution of citric acid (C₆H₈O₇)
  • only a few drops will likely be required

Store at 4^oC

Caution: DMSO can pass through the skin rapidly and may be genetically toxic - wear latex gloves when preparing and working with the citrate buffer.